Zone of Inhibition Kirby-Bauer Test

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Given below is general information pertaining to the Kirby-Bauer Test:


The Kirby-Bauer Zone of Inhibition test method is a qualitatively test to check the action of an antimicrobial agent and is read as the zone of inhibition exerted in an agar medium plate.

The Kirby-Bauer Test method, also known as the Agar Disk Diffusion Test is typically used for screening the antimicrobial activity for variety of samples like pharmaceuticals, intermediates, New Chemical Entities (NCE), Herbal products, Chemicals, etc. 

The bacteria against which test has to be performed is grown overnight. A suspension of this culture is spread uniformly on to the surface of an appropriate agar medium in a Petri dish. The antimicrobial agent is then applied on the inoculated Petri dish. This is followed by incubation at proper temperature and duration.

After incubation the Petri dishes are observed for growth and zone of inhibition. The diameter of the zone of inhibition is considered proportional to the activity of the antimicrobial substance present in the sample or product.

In addition to regular testing, we also conduct studies on new compounds and formulations, to help determine if a particular product holds promise as an antimicrobial agent and what would be the spectrum of activity. In such studies, the test matrix and the microbial cultures are selected as per client requirement or the end use of the compound being designed.

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