Probiotic Formulations Bioassay

Probiotic Formulations and Analysis:

Shubhda Research Institute
Complete solutions for all your Microbiology testing needs! 

Given below is general information pertaining to the Probiotic Test: 


Probiotics are the beneficial bacteria in the human gut. There are many formulations, foods and beverages which are containing different combinations of probiotic microbes. We have long standing experience in testing and bioassays of Probiotic formulations.

  1. Enumeration of probiotics in pure or mixed population of a pharmaceutical or nutritional support formulation – either as a capsule, tablet, sachet or liquid or bulk blend intermediates.
  2. Stability of Probiotic formulations – conducting accelerated stability studies for six months as per ICH guidelines.
  3. pH resistance / tolerance of probiotic formulations
  4. Characterization of probiotics used in a formulation as separate culture at species level.
  5. Pre and post lyophilization survival / viability test of probiotics to determine the efficiency of the process.
  6. Verification of label claim for probiotic market samples – enumeration and identification.
  7. Characterization of probiotics by morphology, biochemical characterization, sugar utilization etc.
  8. Identification up to species level by genotypic method.
  9. Determination of the mole % G + C content of DNA of the probiotic culture.
  10. DNA-DNA hybridization study between same species but different strains for comparison of homology between two comparative DNA sequences based on % match. 

The probiotics tested usually belong to genus Lactobacillus, Bifidobacterium, Bacillus, Saccharomyces, clostridium and Streptococcus species.

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Simulation Studies

Shubhda Research Institute
Complete solutions for all your Microbiology testing needs! 

Given below is general information pertaining to the Simulation Studies: 


Shubhda Research institute takes pride in stating that we have the expertise in conducting in-vitro simulation studies for different applications.
After a new formulation is established and characterized, the product or formulation is taken through or exposed to near actual life conditions. The idea of simulation studies is to mimic the real-life conditions in vitro. The data obtained is useful for scientists or R&D formulation for doing necessary changes, improvements or enhancements in the products. In addition to this, such simulation studies are a confidence building exercise about the product efficacy and form a part of Standardization data.
One example to cite is simulation studies on catheters by exposing the catheters to artificial urine at different temperatures, agitation and varying the contact time. After simulating the real life conditions for a specified duration the antimicrobial activity is tested by suitable ASTM or equivalent method. The microbial count between the initial and different time intervals is compared so as to find the effect of urine contact with respect to reduced antimicrobial activity if any.
Another example of Simulation study is of finding the effect on a probiotic formulation when gut conditions are simulated at acidic and bile salts in stomach and alkaline pH of intestine along with gentle movement and time factoring. Assessment of probiotic viability of different groups in the formulation, enumeration of various genus, and their survival ability at different stages of simulation with time factor are studied and reported.
Simulation studies are set up along with controls which are developed specifically for a study and those give the required baseline to validate the testing.

For a Quote or any clarification, Contact us now!

Probiotic Formulations Bioassay
  • Bioassays
  • pH Simulation studies
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Probiotic Formulations Bioassay
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