Minimum Inhibitory Concentration (MIC)

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Given below is general information pertaining to the MIC Test:


The Minimum Inhibitory Concentration Test (MIC test) is a quick and reproducible test for intended for screening the antimicrobial activity in liquid condition. The test can also differentiate between the “static” or the “cidal” activity. The MIC test can be performed on variety of samples with different combinations of test parameters and many cultures can be used. 
A pure culture suspension is prepared and cell concentration adjusted to desired level. The antimicrobial sample is dissolved in suitable solvent or diluted directly if already a liquid. A test matrix is generated wherein there are several pre-decided dilutions of the antimicrobial test sample. The culture suspension is added into the test matrix and this mixture is then incubated at appropriate conditions. At the end of incubation period, observations are made to check for growth or no-growth in the test matrix. The last set in the dilution that does not exhibit visible growth is usually considered as the Minimum Inhibitory Concentration (MIC) of the antimicrobial sample. The break point is determined and MIC is interpreted.
The typical test method used may be the Macro-broth or Micro-broth dilution method based on the CLSI standards. In some case the Agar Dilution method is also used.

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