ISO 846

Plastics – Evaluation of action of microorganisms

Shubhda Research Institute
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Given below is general information pertaining to the ISO 846 Test: 


This is an test method for evaluating the antibacterial activity of treated plastic materials.

  1. In this test, the plastic test pieces are placed onto the surface of an agar plate which has been seeded with the test microorganism (Pseudomonas aeruginosa or other bacteria or fungi) and then additionally covered with a layer of inoculated molten agar.
  2. The plates are incubated and monitored for 4 weeks to check for microbial growth. For growth, the microorganisms must use the plastic as their food source.
  3. Test is set up in triplicate.

Typical test size of material for testing is 50 mm squares. Appropriate controls are run so as to validate the experiment.

Many microorganisms are used in the method in addition to the recommended P. aeruginosa. We have several cultures in our culture collection for the purpose. Custom modifications in inoculum and extended time are also done at sponsor’s request.

TAT: 4-5 weeks.

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