ISO 22196

Measurement of antibacterial activity on plastics and other non-porous surfaces

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Given below is general information pertaining to the ISO 22196 Test: 


This is an test method for evaluating the antibacterial activity of treated plastic materials. This method is essentially similar to JIS Z2801 test method.

  1. The culture suspension is adjusted to specified cell concentration.
  2. The culture is applied on the test surface and film is placed so as to create a thin uniform layer of the inoculum on the test surface.
  3. The inoculated plates are incubated at body temperature and high humidity for different time periods 1,8,12 or 24h depending on client’s requirement.
  4. After incubation the inoculated culture is extracted by a neutralizing medium.
  5. Comparison between the “zero h” bacterial count and the count after incubation is noted and reduction in the count is calculated represented as log reduction of percentage reduction.

Test is run in triplicates. Appropriate controls are set up in parallel. The controls must pass at each stage without which the experiment stands invalid and testing will have to be repeated. The method includes a “pass/fail” criterion for the calculated levels of antimicrobial activity observed in the test samples. 

TAT: 8-10 working days.

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