ASTM E 2471

Standard Test Method for Using Seeded-Agar for the Screening Assessment of Antimicrobial Activity In Carpets

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Given below is general information pertaining to the ASTM E 2471 Test: 


The ASTM E2471 test method qualitatively evaluates antibacterial and antifungal activity of a carpet at fiber layer and at the primary backing layer when challenged with the test microorganisms.

  1. In this ASTM E 2471, small rectangular pieces of carpet are cut.
  2. Half the portion of the cut piece is shaved to remove the fibers.
  3. Test culture suspension is prepared calibrated to specified concentration.
  4. Recommended growth medium is inoculated with the test culture and mixed by wrist action.
  5. The carpet pieces are immersed in the seeded molten agar medium.
  6. The carpet pieces are kept in Petri dish and additional molten seeded medium is poured into it to surround the carpet piece.
  7. The Petri dishes are incubated at appropriate temperatures.
  8. Observe the results visually and microscopically to check for inhibition

ASTM E 2471 provides for rapid screening of antimicrobial treatments on the carpet face fiber or incorporated at the backing structure of the carpet. The test method has the advantage of mimicking the actual use conditions of carpet.

The sample requirements are - 3 test pieces, 5 x 5 cm with each piece labeled or in a bag that is labeled. An untreated control sample is also required and is considered an additional test sample.

 10-12 working days.

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