The purpose and importance of this ASTM E 2315 test or also known as the Liquid Suspension Time-Kill Test, is that it can used to evaluate the decrease of a microbial population (test organism) after exposure to certain Disinfectant products and materials. Duplicate samples are inoculated with the challenge microorganism and the changes in the inoculum population are evaluated at defined time points. The time selection is dependent on the type and intended use of the test material.
Variables in the test are required to be standardized. The variables cover organism selection, inoculums preparation, sampling times and temperatures. Numbers of microorganisms in the reaction vessel are plotted over time. Neutralization controls are run as appropriate.
The “death curve” i.e. the kill effect of a disinfectant product on a particular microorganism with respect to time can also be studied by exposing microorganisms to the disinfectant. In these cases, attempt is made so as to mimic the real work situation, when using the disinfectant.
TAT: 5 - 10 working days.
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