ASTM E 2180

Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials

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Given below is general information pertaining to the ASTM E 2180 Test: 


  1. In this test method, a sterile molten agar is inoculated with a test organism and then the agar slurry is spread onto the surface of the test pieces as thin layer.
  2. Minimum triplicate samples are set up with appropriate untreated control pieces.
  3. After specified contact time (normally 24h), surviving bacteria are recovered by elution using neutralizing medium.
  4. The bacterial count is determined by serial dilution, plating and incubation.
  5. Bacterial colony counts (cfu) reductions between treated versus untreated controls is calculated and represented as log reduction or percentage.
  6. Different contact times can be used as per client’s request.

Sample requirement is 3" x 3" test pieces for each time point. The test requires zero h and the specified time periods like e.g. 1 hour, 8 hours, 24 hours etc. The test is recommended to be run in triplicate for each sample and each time point. 

Typical test microorganisms used are Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae. Other microorganisms including bacteria, yeast or fungi may also be used as per client or product requirement. 

 8-10 working days 

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