The ASTM E2149 test method is designed to evaluate the antimicrobial activity of non-leaching (non-water soluble) antimicrobials surfaces made of plastic, rubber, silicone and treated fabric material.
ASTM E 2149 is one of the only ways to test an irregularly shaped antimicrobial object, such as a thread, powder, 3D molded plastic.
If an antimicrobial object is a flat panel or has a plain surface, then JIS Z 2801 / ISO 22196 may be more suitable method of choice.
- In this test method, appropriately standardized concentration of freshly cultured microorganism is prepared.
- Experiment is set up by suspending the treated (test material) and untreated (Blank Control) material in separate sterile jars.
- The jars are agitated on a reciprocating shaker for proper and constant contact between the suspended microbial culture and the sample.
- Microbial concentrations in the treated and untreated samples are determined at “zero” as well as 1 or 24 h contact time or time specified by the client.
- The reduction in bacterial population between the jar that contained the antimicrobial product to the jar which had the Blank Control (untreated) is determined.
- The result is represented as log reduction or percentage reduction.
- Interpretation of antimicrobial efficacy is product dependent and final decision lies with the client.
Most commonly used bacteria for ASTM E 2149 are Staphylococcus aureus, E. coli or Klebsiella pneumoniae. However, other bacteria are also used as per client requirement.
A sample requirement for 1 test piece varies from 0.5 to 2 grams, but various substrates can be used, i.e. metal parts, concrete pieces, fabric, polymers, etc.
The ASTM E2149 method is a quantitative test method widely used in the industry.
TAT: 8-10 working days.
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