ASTM E 2149

Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Condition (Dynamic Shake Flask Test Method)

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Given below is general information pertaining to the ASTM E 2149 Test: 


The ASTM E2149 test method is designed to evaluate the antimicrobial activity of non-leaching (non-water soluble) antimicrobials surfaces made of plastic, rubber, silicone and treated fabric material.
ASTM E 2149 is one of the only ways to test an irregularly shaped antimicrobial object, such as a thread, powder, 3D molded plastic.
If an antimicrobial object is a flat panel or has a plain surface, then JIS Z 2801 / ISO 22196 may be more suitable method of choice.  

  1. In this test method, appropriately standardized concentration of freshly cultured microorganism is prepared.
  2. Experiment is set up by suspending the treated (test material) and untreated (Blank Control) material in separate sterile jars.
  3. The jars are agitated on a reciprocating shaker for proper and constant contact between the suspended microbial culture and the sample.
  4. Microbial concentrations in the treated and untreated samples are determined at “zero” as well as 1 or 24 h contact time or time specified by the client.
  5. The reduction in bacterial population between the jar that contained the antimicrobial product to the jar which had the Blank Control (untreated) is determined.
  6. The result is represented as log reduction or percentage reduction.
  7. Interpretation of antimicrobial efficacy is product dependent and final decision lies with the client. 

Most commonly used bacteria for ASTM E 2149 are Staphylococcus aureus, E. coli or Klebsiella pneumoniae. However, other bacteria are also used as per client requirement.
A sample requirement for 1 test piece varies from 0.5 to 2 grams, but various substrates can be used, i.e. metal parts, concrete pieces, fabric, polymers, etc.

The ASTM E2149 method is a quantitative test method widely used in the industry. 

 8-10 working days. 

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