The ASTM E1153 test method determines the efficacy of sanitizing agents on various inanimate surfaces (e.g. floors, counters and other routinely cleaned areas or materials), where bacteria are dried onto the test surface and then the sanitizer is applied on to it. The test method validates the sanitizing procedure for clean rooms or aseptic areas.
This method is quantitative test method, giving the log reduction or percentage reduction of the bacterial population on application of the disinfectant with respect to time.
- In the ASTM E1153 method, the test culture is prepared by repeated subculturing for 4-5 days.
- Specific volume of the culture suspension calibrated to specified cell concentration is dispensed onto test surfaces and allowed to dry.
- Controls are run along with the test to determine the initial microbial count.
- At the end of the contact time, the reaction between microorganisms and the inanimate contact sanitizer is arrested by adding a neutralizer solution.
- Aliquots of this mixture of microorganism, product and neutralizer are serially diluted and the microbial population enumerated using standard microbiological techniques.
- The difference between the controls i.e. the initial microbial or bacterial count and after the treatment with sanitizer and specified contact time indicates the efficacy of the sanitizer.
- The data is represented as log reduction of percentage reduction of microorganisms due to the effect of the sanitizer.
The ASTM E1153 test may be used to find the relative time kill effect or death curve using this test method. Short contact times can also be checked by this method, to determine the disinfectant efficiency.
TAT: 2-3 weeks.
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