ASTM D 4300

Standard Test Methods for Ability of Adhesive Films to Support or Resist the Growth of Fungi.

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Given below is general information pertaining to the ASTM 4300 Test: 

This method tests the ability of adhesive films to inhibit or support the growth of selected fungal species on agar growth medium. The agar plate provides the means of testing the films on two agar substrates, one which promotes microbial growth while the other does not. 

The cultures recommended are: Aspergillus niger, Aureobasidium pullulans, Chaetomium globosum, Gliocladium virens and Penicillium pinophilum. They may be used singly or in combination.
The adhesive sample is prepared by applying the sample onto the fiber glass disk and drying overnight.
The spore suspension is spread uniformly on to the agar surface. The sample coated fiber disc is placed on to the inoculated surface and incubated.
After incubation for appropriate number of days (depending on the fungi and media used) the plates are observed for inhibition and data recorded as per the grading system outlined in the standard.

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