AOAC “Use Dilution Test”
Shubhda Research Institute
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Given below is general information pertaining to the AOAC Use Dilution Test:

The AOAC “Use Dilution Test” method is used to substantiate claims for dilutable disinfectants for their antimicrobial action.

Bacteria like Salmonella, S aureus and P. aeruginosa are covered under these tests.

  1. The test method design remains similar, which involves application of 48 h old bacterial cultures onto sterile penicylinders.
  2. In some case organic soil load, to simulate real conditions is also used.
  3. The cultures are dried onto the surface.
  4. The inoculated and dried cylinders pieces are then individually aseptically transferred into tubes containing 10 ml of disinfectant. 
  5. After specified contact time at ambient (~25 °C), the cylinders are individually transferred to sterile jars and neutralizing growth medium, which neutralizes the active ingredient of the disinfectant and the same time allows growth of the test microorganism if viable cells are present.
  6. The tubes containing the cylinders and neutralizing growth medium are incubated for 48h to check for growth.
  7. The number of tubes showing growth of target microorganism is recorded.

For a set 10 to pass, all samples should exhibit no growth. For a set of 60, pass is when 59 jars show no growth.

Turnaround Time:
5 working days.

If your firm wants to run the AOAC tests, simply Contact our Lab today!

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