AOAC 961.02

Antimicrobial Testing of Germicidal Spray Products

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Given below is general information pertaining to the AOAC 91.02 Test: 


The AOAC 961.02 test method is used to substantiate claims for disinfectants, particularly for “ready-to-use” disinfectants delivered to a surface by aerosol or trigger sprays. This test is a "high-level" test for spray disinfectants, meaning that they must have appreciable biocidal (germ-killing) activity on a relatively short (<10 minutes) time frame to "pass" the test.

  1. The test method involves application of 48 h old bacterial cultures onto sterile glass surface. In some case organic soil load, to simulate real conditions is also used.
  2. The cultures are dried onto the glass surface.
  3. The inoculated and dried glass pieces are then individually sprayed in accordance with the manufacturer’s instructions.
  4. After specified contact time, the glass plates are transferred to sterile jars and neutralizing medium is added into it.
  5. The contact time used can be very short and up to 10 minutes.
  6. The jar along with the inoculated and sprayed glass piece is incubated for 48h to check for growth.
  7. The number of tubes showing growth of target microorganism is recorded.
  8. For a set 10 to pass, all samples should exhibit no growth. For a set of 60, pass is when 59 jars show no growth.

TAT: 5 working days.

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