AATCC 90 ( withdrawn )

Antibacterial Activity Assessment of Textile Materials: Agar Plate Method

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Given below is general information pertaining to the AATCC 90 Test: 


The agar diffusion test is used to determine the bacteriostatic or fungistatic activity of a solid material such as textiles, plastics, coated metals, etc. which contain an antimicrobial or has been treated with an antimicrobial agent.

  1. A suspension of 18-24 h bacterial culture is prepared and further calibrated to specified concentration.
  2. A double layered agar system is prepared where the top layer is seeded with the bacterial inoculum.
  3. Sample discs are cut out ~20mm aseptically using sterile pincette. The disc is placed on the seeded agar surface and pressed slightly avoiding any bubble formation below the disc.
  4. The plates are incubated at 37 °C for 24 for bacteria and 25 °C for fungus for 3-4 days.
  5. Negative control, Growth control and sterility controls are set up in parallel.
  6. After incubation zone of inhibitions is seen are recorded in mm.
  7.  For samples with poor diffusion characteristics, the disc is gently lifted and the surface below is observed for growth or no-growth.

The AATCC 90 is quick qualitative test which can detect the antimicrobial activity against bacteria and fungi. It is widely used test for primary screening of newly developed materials.

 3-5 days for bacteria and 8 days for fungi.

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