The agar diffusion test is used to determine the bacteriostatic or fungistatic activity of a solid material such as textiles, plastics, coated metals, etc. which contain an antimicrobial or has been treated with an antimicrobial agent.
- A suspension of 18-24 h bacterial culture is prepared and further calibrated to specified concentration.
- A double layered agar system is prepared where the top layer is seeded with the bacterial inoculum.
- Sample discs are cut out ~20mm aseptically using sterile pincette. The disc is placed on the seeded agar surface and pressed slightly avoiding any bubble formation below the disc.
- The plates are incubated at 37 °C for 24 for bacteria and 25 °C for fungus for 3-4 days.
- Negative control, Growth control and sterility controls are set up in parallel.
- After incubation zone of inhibitions is seen are recorded in mm.
- For samples with poor diffusion characteristics, the disc is gently lifted and the surface below is observed for growth or no-growth.
The AATCC 90 is quick qualitative test which can detect the antimicrobial activity against bacteria and fungi. It is widely used test for primary screening of newly developed materials.
TAT: 3-5 days for bacteria and 8 days for fungi.
For a Quote or any clarification, Contact Us now!
