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AATCC 174

Antimicrobial Activity Assessment of New Carpets

Shubhda Research Institute
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Given below is general information pertaining to the AATCC 174 Test: 

 


  1. AATCC 174 Part-1: A qualitative antibacterial assessment.
  2. AATCC 174 Part-2: A quantitative antibacterial assessment.
  3. AATCC 174 Part-3: A qualitative antifungal assessmen

In the AATCC 174 Part-1, the bacterial culture is streaked on to the surface of the agar medium and the pre-cut carpet piece is placed over the streak and pressed gently.  This is done for the fibers and also separately for the carpet backing. No growth or growth adjacent to the carpet on the line of streak indicates whether the carpet has any antibacterial activity. This method is a rapid screening method for R&D or trial batches.
In the AATCC 174 Part-2, the test carpets are inoculated with test microorganisms. The concentration of microorganisms in the sample is determined by plate count method. After exporure time, the bacteria are separated from the carpet material by using a neutralizing medium. The number of bacteria in this extracted liquid is determined. The reduction in number of bacteria when compared between the initial “Zero h” count and count after incubation is calculated and represented as log reduction or percentage reduction. This constitutes the quantitative method.

In the AATCC 174 Part-3, the fungal spore suspension is applied on to the surface of an agar medium in a petri dish and a disc of the carpet is placed on to the surface of the agar. Testing is done using both the backing as well as the fibers. The plates are incubated for up to 7 days and the growth free zone if any is noted from the part which is in contact with the agar. 
TAT: 5-7 working days for bacteria and 3-4 weeks for fungus.

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