Antibacterial activity assessment of textile materials: Parallel Streak Method

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Given below is general information pertaining to the AATCC 147 Test: 


This is qualitative test to detect bacteriostatic activity on textile materials. Antimicrobial agents of migratory or diffusible nature are readily evaluated by this method.

  1. Agar petri plates are prepared.
  2. Test culture suspension is streaked in parallel on to the agar surface.
  3. Fabric samples are placed on parallel streaks on test culture.
  4. The pteri plates are then incubated.
  5. Observations are done at 24h for zone of inhibition or no growth in area of contact or growth below and around.
  6. Untreated control and treated samples are set up in parallel with different bacteria.

This is a widely used method for initial screening of different antimicrobial agents applied on textile materials.

Bacteria recommended are S. aureus and K. pneumoniae. However, most clients request for a larger number of cultures and strains to be used, as this test is basically a screening test, which gives an idea of the spectrum of activity.
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